Fig. 5: EPA regulates F-actin organization and cell mechanical properties in CD44 ligand-treated porcine chondrocytes. | Experimental & Molecular Medicine

Fig. 5: EPA regulates F-actin organization and cell mechanical properties in CD44 ligand-treated porcine chondrocytes.

From: Eicosapentaenoic acid restores inflammation-induced changes in chondrocyte mechanics by suppressing the NF-κB p65/CD44 signaling pathway and attenuates osteoarthritis

Fig. 5

a Representative co-immunostaining images of F-actin and CD44 in A6-stimulated porcine chondrocytes pretreated with or without EPA. Scale bar, 20 μm. b–d Quantification of fluorescence intensity (b and c) of F-actin and CD44 and cell circularity (d) in A6-stimulated porcine chondrocytes pretreated with or without EPA. e–j Quantification of Young’s modulus in CD44 ligand-stimulated porcine chondrocytes pretreated with or without EPA. k A schematic illustration of EPA–HA injections. l Size distribution of EPA emulsion in aqueous suspensions. m, n Dynamic viscosity (m) and dynamic shear modulus (n) of intraarticular HA injections and EPA–HA injections. ●, storage modulus (G′) of HA; ○, loss modulus (G″) of HA; ▲, storage modulus (G′) of EPA–HA; △, loss modulus (G″) of EPA–HA. Data are presented as mean ± s.d. n = 50 (b–d) and 40 (e–j). Statistical analysis was performed using one-way ANOVA with Tukey’s multiple-comparisons test. *P < 0.05, **P < 0.01, ***P < 0.001.

Back to article page