Fig. 1: Reduction of PCAF in PTCs during the progression of renal fibrosis. | Experimental & Molecular Medicine

Fig. 1: Reduction of PCAF in PTCs during the progression of renal fibrosis.

From: Loss of PCAF in proximal tubular cells exacerbates renal fibrosis by promoting partial epithelial-to-mesenchymal transition

Fig. 1

a, mRNA levels of PCAF in the kidneys of patients with diabetes mellitus were analyzed using publicly available data, GSE142025 (early DN, n = 6; advanced DN, n = 22; control, n = 9). b, Representative images of MTS and immunohistochemistry (IHC) for α-SMA and PCAF in the kidney samples from the UUO-induced renal fibrosis model (sham, n = 4; UUO, n = 5). c, Protein levels of PCAF in kidney tissues from the UUO-induced renal fibrosis model. d, Representative IHC and MTS images showing the time-dependent PCAF expression in AQP1-positive cells in the kidney samples from the UUO-induced renal fibrosis model post-UUO surgery. The dotted lines represent individual proximal tubule units. e, Evaluation of PCAF expression in AQP1-positive cells using H-scoring (n = 3 per group). f, Representative images of co-immunofluorescence (IF) for PCAF and AQP1 in the kidney samples from the UUO-induced renal fibrosis model (n = 5 per group). The dotted lines represent individual proximal tubule units. The graph illustrates the fluorescence intensity of PCAF in AQP1-positive cells. Data are presented as mean ± s.e.m., *P < 0.05 **P < 0.01, ***P < 0.001, ****P < 0.0001 by t-test for two groups and ordinary one-way ANOVA for multiple groups.

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