Fig. 5: Adenovirus-mediated PCAF gene delivery attenuates renal fibrosis by inhibiting EMT progression. | Experimental & Molecular Medicine

Fig. 5: Adenovirus-mediated PCAF gene delivery attenuates renal fibrosis by inhibiting EMT progression.

From: Loss of PCAF in proximal tubular cells exacerbates renal fibrosis by promoting partial epithelial-to-mesenchymal transition

Fig. 5

a, A schematic diagram illustrating the mouse experiment. Ad5-PCAF was administered via intracapsular injection into the kidney simultaneously with UUO surgery, and the mice were euthanized 7 days later. b, Protein levels of PCAF and fibrotic markers in kidney tissues from the UUO-induced renal fibrosis model with or without PCAF overexpression. c, Representative images of MTS, Sirius Red staining and immunohistochemistry (IHC) for α-SMA in the kidney samples from the UUO-induced renal fibrosis model with or without PCAF overexpression. d, Quantification of the fibrotic area and the DAB-positive area of α-SMA from histological staining images (n = 4 per group). e, mRNA levels of fibrosis-related genes in the UUO-induced renal fibrosis model with or without PCAF overexpression (n = 4 per group). f, mRNA levels of EMT signature genes in the UUO-induced renal fibrosis model with or without PCAF overexpression (n = 4 per group). Data are presented as mean ± s.e.m., *P < 0.05 **P < 0.01, ***P < 0.001, ****P < 0.0001 by t-test for two groups and ordinary one-way ANOVA for multiple groups.

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