Fig. 5: DBC1 is required for efficient chromatin binding of HSF1 and SE-associated HSF1 target gene expression.

a Left: heat maps of downregulated and upregulated HSF1 ChIP-seq signals in SM1 versus SM1-D1KO cells. Right: plots of the average HSF1 ChIP-seq signals at downregulated and upregulated regions. b Left: a Venn diagram showing HSF1 positive direct targets with lost HSF1 binding in SM1 versus SM1-D1KO cells. Middle: a plot of the average HSF1 ChIP-seq signals and heat maps of HSF1 signals on HSF1 positive direct targets with lost HSF1 binding in SM1 versus SM1-H1KO or SM1-D1KO cells. Right: heat maps showing changes in the expression of HSF1 positive direct targets with lost HSF1 binding in SM1 versus SM1-H1KO or SM1-D1KO cells. c A GSEA showing the negative enrichment of HSF1 positive direct targets with lost HSF1 binding in SM1-D1KO compared with SM1 cells. d Heat maps of DBC1 ChIP-seq signals in SM1 versus SM1-D1KO cells. e Left: a Venn diagram showing the overlap of DBC1-regulated genes and DBC1-bound genes in SM1 cells. Right: heat maps showing changes in DBC1 direct target gene expression and DBC1 ChIP-seq signals in SM1 versus SM1-D1KO cells. f A Venn diagram showing HSF1–DBC1 direct cotargets in SM1 cells. g Scatter plots of a Pearson correlation between log₂(fold change) (log₂FC) in expression levels of HSF1–DBC1 direct cotargets in SM1 cells upon H1KO and D1KO. h Scatter plots showing changes in H3K27ac ChIP-seq signals in 22RV1 versus SM1 cells. i Ranked plots of SEs and TEs defined on the basis of H3K27ac ChIP-seq in SM1 and SM1-D1KO cells. j Stacked bar charts showing the overlap of H3K27ac-defined TEs and SEs with HSF1 and/or DBC1. k A Venn diagram showing the overlap of SE-associated genes with DBC1-HSF1 direct cotargets in SM1 cells. SE-associated HSF1–DBC1 direct cotargets include many metastasis-associated genes, which are listed in the box. l Left: heat maps of downregulated and upregulated H3K27ac ChIP-seq signals in SM1 versus SM1-D1KO cells. Right: plots of average H3K27ac signals at downregulated and upregulated regions. m Dot plots showing log₁₀FCs in gene expression that are regulated by lost SEs in SM1 cells upon D1KO or H1KO. The red lines indicate the median FC values. n Snapshots of ChIP-seq tracks for HSF1, DBC1 and H3K27ac and RNA-seq tracks at the MMP11 SE in SM1, SM1-H1KO and SM1-D1KO cells.