Fig. 1: Targeting LUBAC does not exert significant inhibitory effects on HCC cell proliferation in vitro. | Experimental & Molecular Medicine

Fig. 1: Targeting LUBAC does not exert significant inhibitory effects on HCC cell proliferation in vitro.

From: The LUBAC subunit HOIL-1 promotes the progression of HBV-associated hepatocellular carcinoma independently of linear ubiquitination

Fig. 1

a The mRNA levels of HOIL-1, HOIP and SHARPIN in the TCGA LIHC cohort. b The immunofluorescence staining of M1-Ubi in HCC and normal samples. Scale bar, 20 μm. c The cell viability of HCC cells treated with different concentrations of HOIPIN-1 for 48 h. d The cell viability of HCC cells treated with different concentrations of HOIPIN-1/sorafenib/HOIPIN-1+sorafenib for 48 h. e, f The colony-forming capacity of 97H (e) and PLC/PRF5 (f) cells treated with different concentrations of HOIPIN-1/sorafenib for 48 h and the quantification of the colony formation. The Mann–Whitney U test was used in a, d, e and f and the Kruskal–Wallis H test in c. Data are shown as mean ± s.d., *P < 0.05, **P < 0.01, ***P < 0.001. NS, not significant; DAPI, 4′,6-diamidino-2-phenylindole.

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