Fig. 5: Knocking down the expression of HOIL-1 inhibits the progression of HBV-HCC in vitro and in vivo. | Experimental & Molecular Medicine

Fig. 5: Knocking down the expression of HOIL-1 inhibits the progression of HBV-HCC in vitro and in vivo.

From: The LUBAC subunit HOIL-1 promotes the progression of HBV-associated hepatocellular carcinoma independently of linear ubiquitination

Fig. 5

a The qRT–PCR analysis of HOIL-1 mRNA expression in HepG2.2.15 cells infected with the HOIL-1-knockdown lentivirus or the control lentivirus. b The effect of HOIL-1 knockdown on cell proliferation was detected by CCK-8. c The effect of HOIL-1 knockdown on cell proliferation was detected by EdU incorporation. d The effect of HOIL-1 knockdown on cell proliferation was detected by colony formation assays. e The distribution of different cell cycle phases in the HOIL-1-knockdown HepG2.2.15 cells. f The subcutaneous xenograft tumors of the indicated cells, n = 6; tumor volumes were measured and recorded twice a week, and a growth curve was plotted. g The representative image of indicated xenograft tumors and the quantification of the wet weight of tumors. h The representative images and quantification of IHC staining of Ki-67. i A western blot of the mesenchymal marker expression. j, k The migration capacities indicated by Transwell (j) and wound-healing (k) assays in HepG2.2.15 cells infected with the HOIL-1-knockdown lentivirus or the control lentivirus. l The representative images and quantification of IHC staining of N-cadherin in the xenograft tumors. Scale bars, 100 μm. m–o The tumorsphere formation assays (m), CD133+ population (n), EdU incorporation (o) of HepG2.2.15 transduced with shHOIL-1 or shCtrl, or LV-HOIL-1. The Mann–Whitney U test was used in a–e and g–i. Data are shown as mean ± s.d., *P < 0.05, **P < 0.01, ***P < 0.001.

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