Fig. 2: NEDD4L mediates FAIM2 degradation by catalyzing K48-linked ubiquitination. | Experimental & Molecular Medicine

Fig. 2: NEDD4L mediates FAIM2 degradation by catalyzing K48-linked ubiquitination.

From: Fas apoptotic inhibitor molecule 2 mitigates metabolic dysfunction-associated fatty liver disease through autophagic CRTC2 degradation

Fig. 2

a Western blot images of FAIM2 expression in mouse hepatocytes treated with PAOA and CHX (50 μM) and MG132 (50 μM), CQ (50 μM) or dimethyl sulfoxide (DMSO). b Ubiquitination assays determining the ubiquitination of FAIM2 in primary hepatocytes. c Schematic illustration showing the process of screening E3 ligases that interact with FAIM2. d Interactions between FAIM2 and TRIM21, NEDD4 and NEDD4L in HEK293T cells. e Western blot images of FAIM2 expression in HEK293T cells transfected with the indicated plasmids for TRIM21, NEDD4L and NEDD4L. f Western blot images of FAIM2 expression in HEK293T cells transfected with increasing amounts of the NEDD4L plasmid. g Western blot images of FAIM2 expression in PAOA-treated and AdNedd4l-infected hepatocytes. h Western blot images of FAIM2 expression in the indicated hepatocytes infected with increasing amounts of the AdNedd4l. i Ubiquitination assays were used to determine the ubiquitination of FAIM2 after transfection with the indicated plasmids. j Screening of the ubiquitination of FAIM2 by NEDD4L with the indicated types of ubiquitin. k Ubiquitination of FAIM2 in HEK293T cells transfected with the indicated plasmids. **P < 0.01. The data are expressed as the means ± s.d. Statistical analysis was carried out via two-tailed Student’s t-tests.

Back to article page