Fig. 6: GC-dependent regulation of GR, Mecp2 and Fkbp5 gene expression and their associated pathways in HT22 cells. | Experimental & Molecular Medicine

Fig. 6: GC-dependent regulation of GR, Mecp2 and Fkbp5 gene expression and their associated pathways in HT22 cells.

From: Repeated treatment with short-term mild stress reverses aging- and stress-induced emotional and social behavioral deficits

Fig. 6

a, b GC effects on the expression of GR, Fkbp5, Fkbp4, Hsp90aa1, Sgk1, Mkp-1 (a), Hdac2, Sirt1 and Mecp2 (b) in HT22 cells (CON) and HT22 cells treated with GC (400 ng ml-1) for 24 h. c GR knockdown effects on the expression of GR, Fkbp5, Sgk1 and Mecp2 in HT22 cells treated with siRNA-control (siCON) or siRNA-GR (siGR) for 24 h. d Hdac2 knockdown effects on the expression of Hdac2, GR, Fkbp5, Sgk1 and Mecp2 in HT22 cells treated with siRNA-control or siRNA-Hdac2 (siHdac2) for 24 h. e, f Mecp2 knockdown effects on the expression of Mecp2, GR, Fkbp5, Fkbp4, Hsp90aa1, Sgk1, Mkp-1 (e), Bdnf and TrkB (f) in HT22 cells treated with siRNA-control or siRNA-Mecp2 (siMecp2) for 24 h. g, h Mecp2 overexpression effects on the expression of Mecp2, GR, Fkbp5, Fkbp4, Hsp90aa1, Sgk1, Mkp-1 (g), Bdnf and TrkB (h) in HT22 cells treated for 24 h with GC (400 ng/ml), or GC plus pCNS-D2-MeCP2 (pMeCP2). HT22 cells were transfected with pMeCP2 and treated with GC 24 h later. i, j Sgk1 knockdown effects on the expression of Sgk1, GR, Fkbp5, Fkbp4, Hsp90aa1, Mkp1, Hdac2, Sirt1, Mecp2 (i), Bdnf and TrkB (j) in HT22 cells treated for 24 h with GC plus siRNA-control, and GC plus siRNA-Sgk1. k, l Fkbp5 knockdown effects on the expression of Fkbp5, GR, Fkbp4, Hsp90aa1, Sgk1, Mkp1, Hdac2, Sirt1, Mecp2 (k), Bdnf and TrkB (l) in HT22 cells treated for 24 h with GC or GC plus siRNA-Fkbp5. m ChIP assay showing Mecp2 binding to the proximal promoter (P1 and P2 regions) of the Fkbp5 gene in HT22 cells treated with GC (400 ng/ml) for 24 h. n A summary of GC-induced changes in the expression of GR, Hdac2, Sgk1, Mkp-1, Mecp2 and Fkbp5 and their functional pathway. Data are presented as mean ± s.e.m. (n = 2–4 wells per group; 2–3 PCR repeats per group). *P < 0.05; **P < 0.01 (Student’s t-test; one-way ANOVA followed by the Newman–Keuls post-hoc test). See Supplementary Data 4 for statistical details.

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