Fig. 3: Unilateral dry eye leads to corneal nerve abnormalities in the contralateral eye of WT but not Trpv1KO mice. | Experimental & Molecular Medicine

Fig. 3: Unilateral dry eye leads to corneal nerve abnormalities in the contralateral eye of WT but not Trpv1KO mice.

From: A transient receptor potential vanilloid 1-dependent corneal–trigeminal neuroinflammatory circuit promotes corneal neuropathy

Fig. 3

a–d The quantification of intraepithelial corneal innervation at three different levels evidenced by anti-tubulin β3 immunostaining of corneal wholemounts after 10 days of DED induction: subapical (a) (the percent area occupied by nerve endings) and midepithelial (b) (count of nerve endings per field) nerve ending density and complexity of subbasal nerves (c) (sum of intersections at all Sholl radii) and representative micrographs (d). The right extraorbital lacrimal gland was excised in WT or Trpv1KO mice of both sexes, leading to unilateral DED. Thus, the right and left eyes are referred to as ipsilateral (Ipsi) and contralateral (Contra), respectively. Sham-operated animals were included as controls (Ct). All experiments were performed twice or more with six mice per group per experiment. To compare means, two-way ANOVA was used with Dunnett’s post hoc test. *P < 0.05, **P < 0.01. ns, not significant.

Back to article page