Fig. 4: Gene expression changes induced by DED in the trigeminal ganglion of WT versus Trpv1KO mice. | Experimental & Molecular Medicine

Fig. 4: Gene expression changes induced by DED in the trigeminal ganglion of WT versus Trpv1KO mice.

From: A transient receptor potential vanilloid 1-dependent corneal–trigeminal neuroinflammatory circuit promotes corneal neuropathy

Fig. 4

a DED was induced surgically by bilateral extraorbital lacrimal gland excision in WT or Trpv1KO mice for 10 days, and then, the trigeminal ganglia were collected for bulk RNA-seq analysis (female mice, n = 3 per group). Sham-operated (Ct) mice were used as controls, and differential gene expression was calculated between same-treatment mice (either Ct or DED) of the two strains. b A principal component analysis plot of the four experimental groups. c Volcano plots of DEGs (fold change >1.2, adjusted P value <0.05) between the same-treatment mice of the two strains. Upregulated genes in WT mice are shown in red, and downregulated genes are shown in blue. d The number of non-DEGs and DEGs that were detected in both WT Ct versus Trpv1KO Ct and WT DED versus Trpv1KO DED (strain-specific), only in WT Ct versus Trpv1KO Ct (Ct-specific) and only in WT DED versus Trpv1KO DED (DED-specific) analyses. e The proportion of DEGs annotated in the Gene Ontology database as inflammatory process- (GO:0006954) and immune response-related (GO:0006955). f The gene set enrichment analysis (Gene Ontology Biological Process) showing the 30 most significantly up- (red) and downregulated (blue) pathways.

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