Fig. 5: Gene expression changes induced by unilateral DED in the contralateral trigeminal ganglion of WT versus Trpv1KO mice. | Experimental & Molecular Medicine

Fig. 5: Gene expression changes induced by unilateral DED in the contralateral trigeminal ganglion of WT versus Trpv1KO mice.

From: A transient receptor potential vanilloid 1-dependent corneal–trigeminal neuroinflammatory circuit promotes corneal neuropathy

Fig. 5

a Unilateral DED was surgically induced in WT or Trpv1KO mice of both sexes by excising only the right extraorbital lacrimal gland. After 10 days, the contralateral (Contra) trigeminal ganglia were collected for bulk RNA-seq analysis (female mice, n = 3 per group). Sham-operated (Ct) mice were used as controls, and differential gene expression was calculated between same-treatment mice (either Ct or DED) of the two strains. b A principal component analysis plot of the four experimental groups. c Volcano plots of DEGs (fold change >1.2, adjusted P value <0.05) between the same-treatment mice of the two strains. Upregulated genes in WT mice are shown in red, and downregulated genes are shown in blue. d The number of non-DEGs and DEGs that were detected in both WT Ct versus Trpv1KO Ct and WT Contra versus Trpv1KO Contra (strain-specific), only in WT Ct versus Trpv1KO Ct (Ct-specific) and only in WT Contra versus Trpv1KO Contra (Contra-specific) analyses. e The proportion of DEGs annotated in the Gene Ontology database as inflammatory process- (GO:0006954) and immune response-related (GO:0006955). f The gene set enrichment analysis (Gene Ontology Biological Process) showing the 30 most significantly up- (red) and downregulated (blue) pathways.

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