Fig. 6 | International Journal of Obesity

Fig. 6

From: Maternal overnutrition programs epigenetic changes in the regulatory regions of hypothalamic Pomc in the offspring of rats

Fig. 6

DNA methylation changes at hypothalamic Pomc regulatory regions in the adult offspring. a Methylation analyzes of hypothalamic Pomc promoter (− 150 bp to TSS) − 135 site (two-way ANOVA: maternal diet effect, ††P = 0.0037; post-hoc Tukey’s test, *P < 0.05 compared with D-LF/O-LF), − 133 site (two-way ANOVA: maternal diet effect, ††P = 0.0086), − 128 site (two-way ANOVA: maternal diet effect, †P = 0.0122; maternal × postweaning diet interaction, P = 0.0228; post-hoc Tukey’s test, *P < 0.05 compared with D-LF/O-LF), − 125 site (two-way ANOVA: maternal diet effect, †P = 0.0280), − 115 site (two-way ANOVA: maternal diet effect, †P = 0.0473), − 113 site (two-way ANOVA: maternal diet effect, ††P = 0.0042) (n = 6 for D-LF/O-LF and D-HF/O-HF, n = 5 for D-HF/O-LF and D-LF/O-HF). b,c Methylation analyzes of neuronal POMC enhancer region 1 and 2 in the adult offspring fed postnatally LF or HF diet nPE1 CpG 4 (two-way ANOVA: postweaning diet effect, ‡P = 0.0185; n = 6), nPE2 CpG 1 (two-way ANOVA: postweaning diet effect, ‡P = 0.0469; n = 5). Data are shown as mean ± SEM. *P < 0.05, **P < 0.01

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