Fig. 3

Differentially methylated sites have a differential effect on mRNA expression. a Methylation and mRNA levels of TBX15, PRDM16, ACLY, GLI2, LSP1, POU3F3, CCL4, and CDKN1A genes in hASCs. Significant DMSs (#p < 0.0001) identified by EWAS are shown (n = 6 patients per group). Verification of mRNA levels of genes associated with the DMSs identified (n = 4 patients per group). Results are shown as mean ± SEM. t test; *p < 0.05, **p < 0.01 vs. lean hASCs. Gene name in green (decreased methylation and increased mRNA expression), in orange (increased methylation and decreased mRNA expression), and in blue (methylation and mRNA expression incongruity) (color figure online)