Table 2 Cardiac fibrosis in 3- and 6-month-old offspring

From: Normalisation of circulating adiponectin levels in obese pregnant mice prevents cardiac dysfunction in adult offspring

 

Con/PBS

Ob/PBS

Ob/ADN

P(ANOVA)

Male offspring

3-month collagen expression

n = 10

n = 14

n = 14

 

 Col1a1

1.00 ± 0.34

1.44 ± 0.34

1.56 ± 0.33

0.49

 Col3a1

1.00 ± 0.23

1.52 ± 0.28

1.27 ± 0.30

0.76c

6-month collagen expression

n = 10

n = 10

n = 9

 

 Col1a1

1.00 ± 0.15

1.65 ± 0.39

0.91 ± 0.14

0.12

 Col3a1

1.00 ± 0.17

1.67 ± 0.40

0.82 ± 0.13

0.08

Collagen content (picrosirius)

n = 6

n = 5

n = 10

 

 % cross-sectional area

5.2 ± 1.2

4.3 ± 1.4

2.9 ± 0.6

0.22

Female offspring

    

3-month collagen expression

n = 7

n = 6

n = 10

 

 Col1a1

1.00 ± 0.19

0.79 ± 0.19

1.68 ± 0.34

0.07

 Col3a1

1.00 ± 0.08a

1.16 ± 0.18a

2.10 ± 0.32b

<0.01

6-month collagen expression

n = 10

n = 10

n = 8

 

 Col1a1

1.00 ± 0.08a

1.43 ± 0.25a

2.63 ± 0.55b

<0.01c

 Col3a1

1.00 ± 0.10a

1.38 ± 0.23ab

1.87 ± 0.30b

0.03

Collagen content (picrosirius)

n = 8

n = 9

n = 7

 

 % cross-sectional area

2.6 ± 0.3a

3.7 ± 0.8b

6.3 ± 0.8b

<0.01

  1. Mean ± SEM relative expression of Col1a1 and Col3a1 and collagen content determined by picrosirius staining in 3- and 6-month-old offspring of control, PBS-infused pregnant mice and obese pregnant mice infused either with PBS or adiponectin from E14.5 to 18.5. Values in bold indicate significant overall effect of maternal treatment group by one-way ANOVA. Values with different superscripts, a or b, are significantly different from one another by pairwise post hoc comparison (P < 0.05, Holm–Sidak test)
  2. ADN adiponectin, ANOVA analysis of variance, Con control, Ob obese, PBS phosphate-buffered saline
  3. cIndicates ANOVA performed on log transformed data. Statistical analysis of collagen content performed on combined 3- and 6-month old offspring
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