Fig. 1

PTX3 expression during adipocyte differentiation and by inflammatory stimulation in 3T3-L1 adipocytes. a The protein expression and secretion of PTX3 and adiponectin in 3T3-L1 cells during adipocyte differentiation. Induction of Ptx3 mRNA (b) and protein (c) expression by 24 h treatment of IFNγ (10 ng/ml), TNFα (1 nM), IL1β (1 nM), and LPS (1 μg/ml) in 3T3-L1 adipocytes. d Time course of PTX3 protein expression and secretion in 3T3-L1 adipocytes by 1 μg/ml of LPS stimulation. e PTX3 protein expression and secretion in 3T3-L1 adipocytes treated with various doses of LPS for 24 h. For cell culture studies, experiments were repeated 2–3 times yielding similar results. All values are mean ± SEM (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001 vs. basal levels