Fig. 2
From: Pentraxin 3 deficiency exacerbates lipopolysaccharide-induced inflammation in adipose tissue
Effect of PTX3 silencing on LPS-induced inflammation in 3T3–L1 adipocytes. a Ptx3 mRNA expression in 3T3-L1 adipocytes. b Protein expression of PTX3 in 3T3-L1 adipocytes. c Oil Red-O staining of 3T3-L1 adipocytes on day 8 of differentiation. d Adipogenic gene expression in 3T3-L1 adipocytes on day 8 of differentiation. Gene expression of inflammatory cytokines e and ECM gene expression f in 3T3-L1 adipocytes by LPS stimulation (1 μg/ml) for 24 h. The experiments were repeated three times. For cell culture studies, experiments were repeated 2–3 times yielding similar results. Data are expressed relative to the values for scrambled cells. The values are mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 vs. Scr; #p < 0.05, ##p < 0.01, ###p < 0.001 vs. control. Scr: scramble; kd: knockdown
