Fig. 1: Nuclear anomalies in buccal cells from participants.

Photomicrographs of exfoliated buccal mucosa cells stained with Feulgen and Light Green and viewed at 1000× magnification under transmitted light. The figure shows examples of normal differentiated cells (top row) compared to cells presenting different types of nuclear abnormalities (rows below) (buccal MNi = cells with micronuclei; PNCs = cells showing poly-nucleation or multiple nuclei; NBUDs/NPBs = cells with nuclear buds and/or nucleoplasmic bridges). Cells were scored and nuclear abnormalities classified according to the criteria defined in the ‘buccal micronucleus cytome assay’ [45].