Fig. 3: Hepatic fibrosis and apoptosis in 12-month-old offspring. | International Journal of Obesity

Fig. 3: Hepatic fibrosis and apoptosis in 12-month-old offspring.

From: Effects of maternal diet-induced obesity on metabolic disorders and age-associated miRNA expression in the liver of male mouse offspring

Fig. 3

Quantification of Picrosirius red staining (a), relative mRNA levels of Col1a1, Col3a1 and Col4a1 (b), representative images of protein expression (c), relative protein expression of p-SAPK/JNK, Bax and Bcl-2 (d), and percentage of TUNEL-positive cells (e) in the liver of the offspring at 12 months of age. CC 12 m: mother received control diet and offspring-fed control diet after weaning up to 52 weeks of age; OC 12 m: mother received obesogenic diet and offspring fed control diet after weaning up to 52 weeks of age. Values are presented as means ± SEM; CC 12 m: n = 7–9 and OC 12 m: n = 8–9 animals from independent litters for each group. Data were analyzed by unpaired student’s t test or Mann–Whitney U test or Kolmogorov–Smirnov test. *p < 0.05 and **p < 0.01.

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