Table 1 Primer sequences used in the qPCR.

From: Effects of maternal diet-induced obesity on metabolic disorders and age-associated miRNA expression in the liver of male mouse offspring

Target genes

Forward sequence (5′-3′)

Reverse sequence (5′-3′)

Col1a1

GAGAGGTGAACAAGGTCCCG

AAACCTCTCTCGCCTCTTGC

Col3a1

TGACTGTCCCACGTAAGCAC

GAGGGCCATAGCTGAACTGA

Col4a1

GGCCCTTCATTAGCAGGTGT

GTGAGGACCAACCGTTAGGG

Map3k14

TGTCTCAAGATTGCCAGCGA

ACTTCCTGTAGTGCCTTGCC

Tgfβ1

CTGCTGACCCCCACTGATAC

GGGCTGATCCCGTTGATTTC

Ndufb8

GGTATGGCGACTACCCGATG

GTACTGCTTCGGACCCACAG

Sdhb

GAGTCGGCCTGCAGTTTCA

GCCAGAGTATTGCCTCCGTT

Uqcrc2

CCGGGTCCTTCTCGAGATTTT

TGCTTCAATCCCACGGGTTA

Co1

CCCAGATATAGCATTCCCACG

ACTGTTCATCCTGTTCCTGC

Co2

ATAACCGAGTCGTTCTGCCAAT

TTTCAGAGCATTGGCCATAGAA

Atp5a1

CCTTGACCTTCCTTTGCGCT

CATTTTTGGAGACCAGTCCCG

Bax

GAGCTGCAGAGGATGATTGC

AAGTAGAAGAGGGCAACCACG

Bcl-2

GCGTCAACAGGGAGATGTCA

TTCCACAAAGGCATCCCAGC

Ampkα1

TTCGGGAAAGTGAAGGTGGG

AGATGGTGTACTGATGACCTGG

Ampkα2

GGAGAACACCAATTGACAGGC

TCTTCAACCCGCCCATGTT

Pde4d

TGTGACATTTTCCAGAATCTGAC

AGGTTCATGTGCTTCGAC

Nd5

ACGAAAATGACCCAGACCTC

GAGATGACAAATCCTGCAAAGATG

Rplp0

AGATTCGGGATATGCTGTTGGC

TCGGGTCCTAGACCAGTGTTC

Housekeepers

Hprt

GGTTAAGCAGTACAGCCCCA

GTCAAGGGCATATCCAACAACA

Ppia

GTCCAGGAATGGCAAGACCA

GGGTAAAATGCCCGCAAGTC

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