Fig. 2: Adipogenic differentiation of SGBS cells.
From: 20 Years with SGBS cells - a versatile in vitro model of human adipocyte biology
SGBS preadipocytes were cultured in differentiation medium for up to 21 days. A Cells were fixed and intracellular lipids were stained using either OilRed O or BODIPY, bar = 100 µm. B–F During the time-course of adipogenic differentiation, expression of key adipogenic marker genes were determined using qRT-PCR (B–E) or Western Blot (F). Data + SEM of n = 3 experiments are shown, (F) one representative blot is shown.
