Fig. 3: Metabolic properties of SGBS preadipocytes and adipocytes.
From: 20 Years with SGBS cells - a versatile in vitro model of human adipocyte biology
A Insulin-dependent 14C-2-deoxyglucose uptake and (B) glucose incorporation into lipids was determined in SGBS cells and primary human adipose-derived stromal cells (diff-hASCs) differentiated for 14 days into adipocytes in vitro. Data +SEM of n = 4–5 experiments are shown, *p < 0.05, **p < 0.01, ****p < 0.0001 (ANOVA). C–E Oxidative and glycolytic activity was determined in SGBS before (d0) and after (d14) adipogenic differentiation. ATP production rates (C, n = 2) were determined from the corresponding oxygen consumption and extracellular acidification rates. Bioenergetic plots of preadipocytes (D) and adipocytes (E) were calculated using metabolic activators and inducers. Data are normalized for DNA content, one representative experiment of two is shown. Glc Glucose, FCCP Carbonyl cyanide-p-trifluoromethoxyphenylhydrazone, AA Antimycin A Rot rotenone, mon monensin.
