Fig. 2: Regulation of genes related to lipid storage and lipogenesis and analysis of predicted upstream regulators. | International Journal of Obesity

Fig. 2: Regulation of genes related to lipid storage and lipogenesis and analysis of predicted upstream regulators.

From: Acute and long-term exercise adaptation of adipose tissue and skeletal muscle in humans: a matched transcriptomics approach after 8-week training-intervention

Fig. 2

Subcutaneous adipose tissue biopsies of participants that underwent an 8-week training intervention program were analyzed. Biopsies were taken before (Baseline n = 11) and after the intervention (Trained n = 12) as well as 60 min after the first (Untrained Acute n = 10) and last acute exercise bout (Trained Acute n = 13). Transcript level of (A) AACS, (B) ACACA, (C) GPAM, (D) INSIG1, (E) IRS1, (F) LDLR, (G) MID1IP1, (H) PNPLA3, (I) PPARG, (J) SREBF1, (K) ELOVL6, (L) FASN, (M) ANGPTL4, (N) ANGPTL8 was compared between each timepoint. Bars represent mean ± SD, individual data points are depicted. Significant differences were assessed using one-way ANOVA with Tukey correction, *p < 0.05, **p < 0.01, ***p < 0.001, n = 10–13 with n = 6 represented in all timepoints. Ingenuity Pathway Analysis software was used to predict the activation (z-score > 2) or inhibition (z-score < −2) of upstream regulators based on transcriptomic changes (FC ≥ 1.2 and limma t-test p < 0.01) after (O) acute untrained exercise (n = 8) and (P) long-term training (n = 9). Stacked bars represent activation z-score and −log10p values. Direction is based on a positive or negative z-sore indicating activated (red) or inhibited (blue) signaling of upstream regulators. Top 20 upstream regulators based on significant p values (p < 0.05) were plotted.

Back to article page