Fig. 4: The rs1777538 T > C variant destabilizes NFIA-AS2 affecting a miRNA target site. | International Journal of Obesity

Fig. 4: The rs1777538 T > C variant destabilizes NFIA-AS2 affecting a miRNA target site.

From: Identification and characterization of the long non-coding RNA NFIA-AS2 as a novel locus for body mass index in American Indians

Fig. 4

A Rs1777538 T > C variant disrupts a miRNA target site. The rs1777538 variant is located within the seed region (underlined) for a putative target site for 3 miRNAs miR4270, miR6754-5p, and miR4441 [figure adapted from lncRNASNP2 database (16)]. The rs1777538 C allele is shown in orange font and underlined. B Both miRNA mimics miR4270 and miR6754 significantly decrease NFIA-AS2 levels in HEK293 cells. Data was normalized with eGFP (transfection efficiency) and TBP. C Relative luciferase activity for the binding of miR4270 and miR6754-5p to NFIA-AS2 in an allele-specific manner. The miRNA target site constructs carrying rs1777538 alleles (C) or (T) were cotransfected with either a human miRNA mimic or a negative control in HEK293 cells. Observed luciferase activity was normalized with respect to the empty vector (E). Results are from 3 independent experiments each done in triplicate and shown as mean + SD. Data points per group are shown as hollow circles. Statistical analysis was carried out by Student’s t test. *P < 0.05; **P ≤ 10−5.

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