Fig. 5: Gene expression analysis for the 13 NFIA-AS2 neighboring genes during brown preadipocyte differentiation.

A Schematic showing PAZ6 brown adipogenesis over a period of 14 days assessed by Oil-O-Red staining at days D0 (brown preadipocytes), D6, D10, and D14 (brown adipocytes). D: induction of differentiation; M: initiation of maturation. B Expression patterns for the NFIA-AS2 neighboring genes during brown adipogenesis. Gene expression was normalized with TBP. Preadipocyte differentiations were repeated 3 times on separate days and each differentiation had 3 replicates. Data is shown as mean + SD. Data points per group are shown as hollow circles. Significance was calculated with respect to D0 using the Student’s t test. *P ≤ 10−3; **P ≤ 10−5.