Fig. 1: Fas regulates p53 protein levels in adipocytes.
From: Expression of p53 in human adipose tissue correlates positively with FAS and BMI
a Left panel: Western blot and quantification of Fas and p53 protein levels in white adipocytes harvested from HFD-fed FasF/F and FasΔadipo mice. n = 6–7 mice per group. **p < 0.01. Right panel: Western blot and quantification of p53 protein levels in subcutaneous white adipocytes treated with or without 0.4 ng/ml FasL for 24 h. n = 5 cell culture wells of 3 independent experiments. *p < 0.05. b Representative Western blot and oxygen consumption rate (OCR; n = 37–41 cell culture wells of 2 independent experiments) in control (Co) and p53-depleted (p53 KO) subcutaneous adipocytes treated with or without 0.4 ng/ml FasL for 72 h. Co vs. Co + FasL, p < 0.001; p53 KO vs. p53 KO + FasL, p = 0.43; Co vs. p53 KO, p < 0.001. c Basal and proton leak-linked respiration calculated from OCR data. *p < 0.05, **p < 0.01, ***p < 0.001. d Scatter plot and correlation coefficient (r) of FAS and p53 mRNA expression in differentiated human mesenchymal stem cells derived from paired femoral (n = 18) and abdominal (n = 16) subcutaneous adipose tissue samples. Statistical tests used: Mann–Whitney (left panel) and Student’s t test (right panel) for (a); two-way ANOVA for (b, c); Spearman correlation for (d).
