Fig. 1: The relationship between Grk5 expression and adiposity.
From: GRK5 is required for adipocyte differentiation through ERK activation
A Eight-week-old male C57BL/6J mice were fed chow or a high fat diet (Envigo #TD 88137, 42% from fat, 0.2% total cholesterol) for 16 weeks and their body weight was measured (n = 5/diet group). B Mice were then fasted for 24 h and their epididymal (Epi) visceral (Vis) white adipose tissue (WAT) and brown adipose tissue (BAT) RNA was extracted and reverse-transcribed into cDNA for real-time PCR quantification of Grk5 normalized to 18 s (endogenous control). C Six-week-old male C57BL/6J mice were fed chow or a high fat diet (Research Diets Inc #D12492, 60% from fat) for 12 weeks and their body composition such as fat mass was quantified by EcoMRI (n = 10/diet group). D Adipocyte fraction and stromal vascular (SV) cell fraction were isolated from the Epi Vis WAT of overnight fasted mice. Both fractions’ RNA was extracted and reverse-transcribed into cDNA for real-time PCR quantification of Grk5 normalized to 18 s (endogenous control). All results are mean ± SEM, presented as the fold change compared to chow-fed mouse group and analyzed using a two-tailed Student’s unpaired t test (A–C), or the fold change compared to chow SV fractions and analyzed using a one-way ANOVA with Sidak multiple comparisons (D).
