Fig. 4: The potential underlying mechanisms of GRK5 in adipocyte differentiation.
From: GRK5 is required for adipocyte differentiation through ERK activation
A Pathway analysis of RNA sequencing data using Limma. B Wildtype (WT) control and GRK5 knockout (KO) 3T3-L1 preadipocytes (n = 2/genotype) were differentiated for 2 days and treated with 1 μg/ml of insulin for 5, 10, and 15 min. Then, the cellular proteins were harvested and subjected to Western blot using anti-GRK5, anti-phosphorylated (p)-ERK, anti-ERK, and anti-GAPDH antibodies.
