Fig. 1: In-vivo study set-up.

A Layout of in-vivo study over 20 week period incorporating male and female C57BL6 mice. Mice purchased at 4 weeks were placed on LFD then shaved at 7 weeks and placed on appropriate HFD (n = 20) or LFD (n = 10). Half of the mice on HFD (n = 10) had UV exposures starting at 8 weeks as described. Study groups were split evenly between male and female mice. B Cages used for exposure of male and female C57BL6 mice in-vivo. Modified cages built in house by engineers prevented mice from laying on top of each other and huddling together, while at the same time allowing mice to view each other meaning stress was reduced. Air holes within cages allowed for air flow and heat dissipation.