Fig. 7: DNA damage assessment of exposure lamp in human skin explant and keratinocyte monolayers. | International Journal of Obesity

Fig. 7: DNA damage assessment of exposure lamp in human skin explant and keratinocyte monolayers.

From: Ultraviolet exposure of mice fed a high fat diet reduces weight gain and markers of liver disease progression

Fig. 7

A DNA damage assessment of primary skin cells. Here Western blot was used for assessing γ-H2AX induction, representing double stranded breaks after UV light exposure in neonatal keratinocyte monolayers (3 different donors) with GAPDH as a loading control. Loaded as positive control first (Biosun (1J/cm2 UV-B 9J UV-A), Cleo light-source (forming experimental group) (UV-A 20 J/cm2 and UV-B 0.4 J/cm2) and negative (unexposed) control. B Assessment of western blotting bands via ImageJ analysis. Here results are represented as fold change with positive, CLEO test and negative respectively. C Cyclobutane pyrimidine dimers (CPDs), denoting charesteristic signiture mutantions induced by UV exposure. CPD staining in explants of neonatal skin sections with positive control (Biosun), Cleo and negative control, white arrows point to examples of CPD staining.

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