Fig. 1: Protocol for isolation of endothelial progenitor cells from solid fractions of human adipose tissue.

Fat tissue fragments (approximately 1–2 g) were minced and digested. The resulting material was filtered and the flow-through, termed SVF-I, was centrifuged. The obtained floating fraction was plated in the presence of culture medium specific for human ASC, as previously described [26]. The digestion product retained by cell strainer, termed SVF-II, was further digested, filtered, and the flow-through was centrifuged. The supernatant obtained was plated in the presence of culture medium specific for human ASC [26]. ASC adipose stem cells, SVF stromal vascular fraction. The figure was created using BioRender Software.