Fig. 5: Expression of specific endothelial cell markers in CD31+ and CD31- cells from SVF-II.

A Cells were fixed and incubated with Von Willebrand Factor (vWF) or VE-Cadherin antibodies, followed by Alexa Fluor (488) anti-rabbit antibody (green) to stain vWF or Alexa Fluor (488) anti-mouse antibody (green) to stain VE Cadherin, respectively. TO-PRO-3 was used to stain nuclei (blue). Scale bar: 47.62 mm. HUVECs were used as positive control. Negative control was represented by cells treated only with secondary antibody. B E-selectin, e-NOS, VEGFR, and CD34 mRNAs were analyzed in CD31+ and CD31- cells from SVF-II by quantitative reverse transcription PCR. All data are presented as mean ± standard error of the mean of three experiments, which were carried out using cells from different human donors. *, p < 0.05 vs HUVECs; #, p < 0.05 vs CD31- cells. HUVECs were used as positive control. 18S was used as loading control. C E-selectin and e-NOS proteins were analyzed in CD31+ and CD31- cells from SVF-II by immunoblotting. All data are presented as mean ± standard error of the mean of at three experiments for E-selectin, and five experiments for e-NOS, which were carried out using cells from different human donors. #, p < 0.05 vs CD31- cells. HUVECs were used as positive control. Beta-actin was used as loading control. HUVECs human umbilical vein endothelial cells, SVF stromal vascular fraction.