Fig. 6: Functional characterization of CD31+ and CD31- cells from SVF-II.
From: Isolation of endothelial progenitor cells from human adipose tissue
A Representative images obtained in light microscopy of tube formation of HUVECs, and SVF-II-derived CD31+ and CD31- cells grown on 3D scaffolds (Geltrex). The quantification of the tube number was performed using CellProfiler (v.4.2.8). *, p < 0.05 vs HUVECs; #, p < 0.05 vs CD31- cells. HUVECs were used as positive control. B Representative images obtained in fluorescence microscopy of acetylated LDL uptake (red) evaluated in HUVECs, and SVF-II-derived CD31+ and CD31- cells. TO-PRO-3 was used to stain nuclei (blue). The quantification of the red perinuclear signal was performed using CellProfiler (v.4.2.8). *, p < 0.05 vs HUVECs; #, p < 0.05 vs CD31- cells. HUVECs were used as positive control. HUVECs human umbilical vein endothelial cells, SVFs stromal vascular fraction.
