Fig. 7
High expression of ADAM9, cathepsin V and kallikrein 5 in oral cancer cell lines Cancer cell lines were cultured in RPMI1640 or DMEM/F12 with 10% fetal bovine serum (FBS). Total protein was collected from cell lines and the expression of ADAM9, cathepsin V or kallikrein 5 was detected via western blot. Intracellular protein expression was analyzed by immunofluorescence. a The expression of kallikrein 5, cathepsin V or ADAM9 protein in cancer cell lines was analyzed by western blot. β-actin was used as a loading control. b Quantitative analysis of western blots. c Intracellular expression (red) of kallikrein 5, cathepsin V or ADAM9 in cancer cells lines as analyzed by immunofluorescence. Blue (nuclear) DAPI stain. Images are representative of three independent experiments. Numerical data are presented as the mean ± standard deviation (SD). HSC-4 and SCC-25 oral squamous carcinoma cell lines, ACC-2 oral adenocarcinoma cell line, PDLCs non-cancer cell line. *P < 0.05 compared with PDLCs. #P < 0.05 compared with PDLCs
