Fig. 2
CAPE induces HO1 gene expression. Murine macrophages and RAW264.7 cells were stimulated overnight with 10 µmol·L−1 CAPE, and gingival fibroblasts were stimulated with 20 µmol·L−1 CAPE. HO1 gene expression was assessed (a), n = 3. HO1 protein expression was assessed by Western blotting in murine macrophages (b). Only relevant signals are shown. The full-length blots are presented in Supplementary Fig. 3. The impact of MAPK signalling was investigated using the respective inhibitors (SP600125 for JNK, SB203580 for p38 and U0126 for ERK) (c), n = 3. HO1 gene expression was further investigated in the Nrf2−/− mice (d), n = 3. Data represent the mean ± SD. *P < 0.05 and **P < 0.01 in the two-tailed Mann-Whitney test
