Fig. 3 | International Journal of Oral Science

Fig. 3

From: Defining a critical period in calvarial development for Hedgehog pathway antagonist-induced frontal bone dysplasia in mice

Fig. 3

Frontal bone development in GD10.5 embryos. Histology of the frontal primordium from E12.5 (a, b), E14.5 (c, d), and E16.5 (e, f) embryos. a, b In GD10.5 embryos, a reduced cell density (double arrow) was observed in the frontal primordium at E12.5. c, d At E14.5, mineralized bone was formed within the frontal bone primordium, but there was less bone matrix (arrow) in GD10.5 embryos. e, f At E16.5, frontal bone formation was visible in the vehicle-exposed and GD10.5 samples (double arrow). In GD10.5 embryos, the frontal bones had a reduced trabecular structure (T) and reduced thickness. g, h 3D images generated from micro-CT scans of E18.5 calvaria. Relative to vehicle-exposed embryos, the frontal bones and parietal bones were smaller with a concomitant increase in the width of the metopic and sagittal sutures in GD10.5 mice. Scale bars = 200 μm in (a–f); 1 mm in (g, h)

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