Fig. 4

Compromised frontal bone formation in GD10.5 embryos at E16.5. Sections were cut through the anterior (a, b), middle (c, d), and posterior (e, f) portions of the eyes. In these Aniline blue-stained sections, the osteoid appears dark blue (dotted black lines). g Total tissue area of frontal bones. h Trabecular area of frontal bones. There was no obvious difference in the areas of the frontal bones or trabecular structure in the anterior portions of the frontal bones between vehicle-exposed and GD10.5 embryos. However, in GD10.5 embryos, the frontal bones were smaller and the trabecular structure considerably looser in the middle and posterior portions of the frontal bones. n = 6 embryos per group. Values are the mean ± SD. Data were analysed using unpaired Student’s t tests. ***P< 0.001. Scale bars = 500 μm