Fig. 5 | International Journal of Oral Science

Fig. 5

From: Defining a critical period in calvarial development for Hedgehog pathway antagonist-induced frontal bone dysplasia in mice

Fig. 5

Expression of Hh signalling pathway effectors in embryos. At E12.5, compared to vehicle-exposed embryos, immunofluorescent staining for Ptch1 (a, b) and Gli1 (e, f) in coronal sections of GD10.5 embryos showed a slight reduction in the frontal bone primordium (dotted white lines), and an even greater decrease was observed in Gli3 expression (i, j) in the frontal bone primordium. At E13.5, Ptch1 (c, d), Gli1 (g, h) and Gli3 (k, l) expression levels were markedly diminished in the frontal bone primordium of GD10.5 embryos compared with vehicle-exposed embryos. Consistent with this finding, the sections in the white boxed areas show lower magnification views of the frontal bone primordium. Arrowheads point to Gli3-negative cells. m There was a significant reduction in p = Ptch1, Gli1 and Gli3 intensity in the frontal bone primordium in GD10.5 embryos in comparison to vehicle-exposed embryos (n = 6 embryos per group from three litters). The values are the mean ± SD. Data were analysed using unpaired Student’s t tests. **P< 0.01; ***P< 0.001. Scale bars = 50 μm

Back to article page