Fig. 7 | International Journal of Oral Science

Fig. 7

From: Defining a critical period in calvarial development for Hedgehog pathway antagonist-induced frontal bone dysplasia in mice

Fig. 7

Reduced cell proliferation and increased cell death within the frontal primordium in GD10.5 embryos. a–h Red outlines indicate the frontal bone primordium. a–d Immunostaining with anti-phosphorylated histone H3 showed a significant decrease in cell proliferation in GD10.5 embryos. e–h Cell death was detected by antibody detecting cleaved caspase3. GD10.5 embryos showed increased cell death. i Statistical analysis of cell proliferation in the frontal bone primordium at E12.5 and E13.5. A significantly lower ratio of cells was observed in GD10.5 at both age stages. j A statistically significant increase in the apoptotic index at E12.5 and E13.5 was noted in the GD10.5 frontal bone primordium. n = 12 for each group at each age. The values are the mean ± SD. Data were analysed using unpaired Student’s t tests. ***P< 0.001. Scale bars = 200 μm

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