Fig. 8 | International Journal of Oral Science

Fig. 8

From: Defining a critical period in calvarial development for Hedgehog pathway antagonist-induced frontal bone dysplasia in mice

Fig. 8

Osteogenic progenitor cell differentiation in the frontal bone primordium. Immunohistochemistry of Runx2 at E13.5 (a, b) and E14.5 (c, d) and Bmp4 at E12.5 (e, f) and E13.5 (g, h) in vehicle-exposed and GD10.5 mice. The insets show higher magnification images of the boxed areas. a–d At E13.5 and E14.5, expression levels of Runx2 in frontal bone primordium (black arrow) were lower in GD10.5 mice. e–h There was no obvious difference in the expression of Bmp4 between vehicle-exposed and GD10.5 mice. i, j Statistical analysis of relative expression levels of Runx2 and Bmp4 (n = 12 for each group at each age). Values are the mean ± SD. Data were analysed using unpaired Student’s t tests. **P< 0.01; ***P< 0.001. Scale bars = 200 μm

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