Table 3 The effects of the lentiviral vector on TRPV1 protein and mRNA expression in the TG of rats
From: Transient receptor potential Vanilloid 1-based gene therapy alleviates orthodontic pain in rats
Items | Time intervals/d | Force + TRPV1 shRNA lentivirus group | Force + blank lentivirus group | P value | ||
|---|---|---|---|---|---|---|
Mean ± SD | 95% CI | Mean ± SD | 95% CI | |||
Protein expression | 0 | 0.334 ± 0.023 | 0.309,0.359 | 0.567 ± 0.085 | 0.471,0.663 | 0.064 |
1 | 0.397 ± 0.090 | 0.295,0.499 | 0.892 ± 0.056 | 0.829,0.955 | < 0.001*** | |
3 | 0.428 ± 0.072 | 0.348,0.508 | 1.254 ± 0.046 | 1.201,1.307 | < 0.001*** | |
5 | 0.254 ± 0.159 | 0.074,0.434 | 0.963 ± 0.111 | 0.838,1.088 | < 0.001*** | |
7 | 0.265 ± 0.171 | 0.071,0.459 | 0.882 ± 0.143 | 0.719,1.045 | < 0.001*** | |
14 | 0.190 ± 0.063 | 0.117,0.263 | 0.758 ± 0.100 | 0.644,0.812 | < 0.001*** | |
mRNA expression | 0 | 0.847 ± 0.361 | 0.437,1.257 | 1.000 ± 0.018 | 0.978,1.022 | 0.999 |
1 | 3.528 ± 0.244 | 3.252,3.804 | 6.309 ± 0.566 | 5.668,6.952 | < 0.001*** | |
3 | 2.808 ± 0.544 | 2.181,3.435 | 5.742 ± 0.889 | 4.737,6.747 | < 0.001*** | |
5 | 2.543 ± 0.684 | 1.769,3.317 | 3.860 ± 0.242 | 3.586,4.134 | 0.016* | |
7 | 1.893 ± 0.464 | 1.368,2.418 | 3.295 ± 0.287 | 2.972,3.618 | 0.009** | |
14 | 1.387 ± 0.027 | 1.130,1.644 | 2.585 ± 0.545 | 1.970,3.200 | 0.033* | |
