Fig. 2

Forced overexpression of Dlx2 enhanced osteogenesis of BMSCs in vitro. a ALP staining was performed on days 7 or 14 after osteogenic induction. Alizarin red staining was carried out after cells were cultured in OIM for 14 or 21 day. b Semi-quantitative analysis of ALP activity in Dlx2-overexpressing BMSCs (over) and control BMSCs (control) after 14-day culture in OIM. c Magnified views of ALP staining and Alizarin red staining in a. Scale bar = 50 μm in all the panels. Left panel, 40-fold magnified image; middle panel, 100-fold magnified image; right panel, 200-fold magnified image