Fig. 1
From: Compliant substratum modulates vinculin expression in focal adhesion plaques in skeletal cells
Substrate elasticity alters vinculin expression in chondrocytes. a Representative CLSM images showing cytoskeletal changes by characterizing F-actin in chondrocytes in response to substrates with different stiffnesses (n = 4). Changes of F-actin in chondrocytes were also presented in the High-Low model in CLSM. b Histogram showing the change in cell spreading area of chondrocytes in response to substrates with different stiffnesses. Statistical data are based on more than 100 cells in each group in four independent experiments (n > 100). *P < 0.05. c Histogram showing the change in F-actin by immunofluorescence intensity in chondrocytes in response to substrates with different stiffnesses. Statistical data are based on more than 100 cells in each group in four independent experiments (n = 4). *P < 0.05. d Western blotting showing the change of vinculin in chondrocytes in response to substrates with different stiffnesses. Quantification was then performed to confirm the changes (n = 3). *P < 0.05. e Representative CLSM images showing the change in vinculin by immunofluorescence staining in chondrocytes in response to substrates with different stiffnesses. f Histogram showing the change in vinculin in (e) chondrocytes in response to substrates with different stiffnesses. Statistical data are based on more than 80 cells in each group in four independent experiments (n = 4). *P < 0.0
