Fig. 2
From: Compliant substratum modulates vinculin expression in focal adhesion plaques in skeletal cells
Substrate elasticity alters vinculin expression in osteoblasts. a Representative CLSM images showing cytoskeletal changes by characterizing F-actin in osteoblasts in response to substrates with different stiffnesses (n = 3). Changes of F-actin in osteoblasts are also presented in the High-Low model in CLSM. b Histogram showing the change in the cell spreading area of osteoblasts in response to substrates with different stiffnesses. Statistical data are based on more than 110 cells in each group in three independent experiments (n = 3). *P < 0.05. c Histogram showing the change of F-actin by immunofluorescence intensity in osteoblasts in response to substrates with different stiffnesses. Statistical data are based on more than 110 cells in each group in three independent experiments (n = 3). *P < 0.05. d Western blotting showing the change of vinculin in osteoblasts in response to substrates with different stiffnesses. Quantification was then performed to confirm the changes (n = 3). *P < 0.05. e Representative CLSM images showing the change in vinculin by immunofluorescence staining in osteoblasts in response to substrates with different stiffnesses. f Histogram showing the change of vinculin in (e) osteoblasts in response to substrates with different stiffnesses. Statistical data are based on more than 100 cells in each group in three independent experiments (n = 3). *P < 0.05
