Fig. 3
From: Compliant substratum modulates vinculin expression in focal adhesion plaques in skeletal cells
Substrate elasticity alters vinculin expression in osteoclasts. a Representative CLSM images showing cytoskeletal changes by characterizing F-actin in osteoclasts in response to substrates with different stiffnesses (n = 5). Changes in F-actin in osteoclasts are also presented in the High-Low model in CLSM. b Histogram showing the change in cell spreading area of osteoclasts in response to substrates with different stiffnesses. Statistical data are based on more than 90 cells in each group in five independent experiments (n = 5). *P < 0.05. c Histogram showing the change of F-actin by immunofluorescence intensity in osteoclasts in response to substrates with different stiffnesses. Statistical data are based on more than 90 cells in each group in five independent experiments (n = 5). *P < 0.05. d Western blotting showing the change in vinculin in osteoclasts in response to substrates with different stiffnesses. Quantification was then performed to confirm the changes (n = 3). *P < 0.05. e Representative CLSM images showing the change in vinculin by immunofluorescence staining in osteoclasts in response to substrates with different stiffnesses. f Histogram showing the change of vinculin in (e) osteoclasts in response to substrates with different stiffnesses. Statistical data are based on more than 90 cells in each group in five independent experiments (n = 5). *P < 0.05
