Fig. 1
From: Filamentation initiated by Cas2 and its association with the acquisition process in cells
The C-terminal 30 amino acids are necessary and sufficient for Cas2Em-initiated filamentation in BL21(DE3). a Representative images of BL21(DE3) cells with expression of the vector control, Cas1Em, Cas2Em and Cas1Em-Cas2Em (scale bar, 25 μm). b Fluorescent images of Cas2Em-initiated filamentation labelled by SYTO 9 (green) and FM 4–64 (magenta) to indicate the DNA and membrane, respectively (scale bar, 5 μm). c The presence (+) and absence (-) of the filamentation phenotype with Cas2Em constructs, including four N-terminal and four C-terminal deletions. d Representative images of BL21(DE3) cells with expression of the vector control, Cas2Em71-101 and Cas2Nm
