Fig. 3

Effects of autophagy activators on autophagy and FN1 expression in SCC-25 cells. a Western blot data for cells treated with the activator rapamycin (200 nmol·L⁻¹). b Bar graphs of the results in (a); the intensity of each protein band was normalized to that of GAPDH. c Western blot data for cells treated with the activator EBSS. d Bar graphs of the results in (c); the intensity of each protein band was normalized to that of GAPDH. e RT-qPCR data of FN1 expression in cells treated with the activator rapamycin (200 nmol·L⁻¹) or EBSS (f). g FN1 expression, as detected by immunofluorescence, in cells treated with the activator rapamycin. h Quantification of the average fluorescence values of single cells in (g). Experiments were repeated twice