Fig. 2 | International Journal of Oral Science

Fig. 2

From: Human β-defensin 3 gene modification promotes the osteogenic differentiation of human periodontal ligament cells and bone repair in periodontitis

Fig. 2

Effects of Ad-hBD3 gene transfection on the osteogenic differentiation of the hPDLCs in an inflammatory environment. hPDLCs were transfected with Ad-hBD3 or NC (Ad-GFP) and treated with or without E. coli LPS. a ALP staining and ALP activity on day 7. b Alizarin red S staining of the mineralized nodules on day 21. c qRT-PCR and d western blot analysis of ALP, Runx2, and COL1 expression on day 7. (***, ###P < 0.001, where triple asterisks (***) indicate a significant difference compared with the NC group, and triple hashes (###) indicate a significant difference compared with the NC+LPS group)

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