Fig. 2 | International Journal of Oral Science

Fig. 2

From: PKD3 promotes metastasis and growth of oral squamous cell carcinoma through positive feedback regulation with PD-L1 and activation of ERK-STAT1/3-EMT signalling

Fig. 2

PKD3 downregulation suppresses the proliferation, migration and invasion of OSCC cells. a Effects of PKD3 on the proliferation of Cal-27 and HSC-4 cells as evaluated using the CCK-8 assay. b Transwell migration assay performed with Cal-27 and HSC-4 cells transfected with shRNA-PKD3 or shRNA-Con plasmids. The number of cells that had migrated per field is shown in the bar graphs; scale bar size = 50 µm. c Transwell invasion assay for the indicated cell lines. The number of cells passing through the Matrigel per field is shown in the bar graphs; scale bar size = 50 µm. Nuclei were stained with DAPI (blue). Red represents the cytoskeleton labelled with 555 phalloidin. Data are presented as the means ± SD (n = 3). **P < 0.01; ***P < 0.001. PKD3, protein kinase D3; CCK-8, Cell Counting Kit-8; OSCC, oral squamous cell carcinoma

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