Fig. 1 | International Journal of Oral Science

Fig. 1

From: Exosomes derived from 3D-cultured MSCs improve therapeutic effects in periodontitis and experimental colitis and restore the Th17 cell/Treg balance in inflamed periodontium

Fig. 1

Characterization of 2D-exos and 3D-exos. a Brightfield images of 3D-cultured or 2D-cultured DPSCs. Scale bar = 100 μm. b TEM images showing the morphology of 2D-exos and 3D-exos. Scale bar = 100 nm. c Flow cytometric analysis showing the expression of CD63 and CD9 in 2D-exos and 3D-exos. d Representative western blot image showing the expression of exosome markers (CD9, CD63, and TSG101) and a cytosolic marker (GM130) in 2D-exos and 3D-exos. e Particle sizes and numbers of 2D-exos and 3D-exos were analyzed by using NTA. f BCA assays determined the yields of 2D-exos and 3D-exos

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