Fig. 3 | International Journal of Oral Science

Fig. 3

From: The osteogenesis of Ginsenoside Rb1 incorporated silk/micro-nano hydroxyapatite/sodium alginate composite scaffolds for calvarial defect

Fig. 3

Roles of ERK and AKT signal paths in the osteogenesis differentiation of BMSCs induced by ginsenoside Rb1. a, c WB of phosphorylated and total ERK and AKT after ginsenoside Rb1 exposure at 0, 15, 30, 60, and 120 min and the treatment of ERK inhibitor PD98059 (20 μmol·L−1, PD in short) or AKT inhibitor LY294002 (20 μmol·L−1, LY in short). b, d Densitometry assay of p-ERK/ERK and p-AKT/AKT expression in western blot assay. e Realtime PCR assay of osteogenesis and angiogenesis gene expression of BMSCs posterior to the exposure to ginsenoside Rb1 with PD and LY. f, g ALP dyeing and quantitation analysis . (*,#P < 0.05; *in contrast to Con group, # in contrast to ginsenoside group)

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